[Histonet] [External] Re: Agarose embedded tissue arrays embedded in paraffin block

[Otto, Christopher M]

I didn't take part in the preparation of the agarose or the processing. Was pretty much just handed the blocks and asked to make slides.

Get Outlook for Android<https://aka.ms/AAb9ysg>
________________________________
From: Jay Lundgren <jaylundgren at gmail.com>
Sent: Friday, May 17, 2024 2:31:44 PM
To: Otto, Christopher M <christopher-otto at uiowa.edu>
Cc: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [External] Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block

You don't often get email from jaylundgren at gmail.com. Learn why this is important<https://aka.ms/LearnAboutSenderIdentification>
Where did the agarose come from?

On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:


Hello everyone!

 I'm having trouble sectioning tissue array blocks where the array is in agarose embedded into a paraffin block.  I've chilled the blocks and I'm sectioning on a rotary microtome, at 5 microns, with a high profile Accuedge blade. The paraffin surrounding the agarose sections normally, but the agarose portion of the block causes the blade to "skip" across it slightly and even chip out as if my blade isn't snug in the blade holder (it is).  If I do get a tiny portion of agarose on my section to float out on the waterbath it flies away (like adding ETOH to a waterbath with sections already on it.)   Everyone I have asked about this says the agarose should section normally with the paraffin like any other FFPE blocks. Any ideas on why this agarose is behaving this way for me?  Thank you in advance!



_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet