[Histonet] Agarose embedded tissue arrays embedded in paraffin block

[Jay Lundgren]

Where did the agarose come from?

On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

>
>
> Hello everyone!
>
>  I'm having trouble sectioning tissue array blocks where the array is in
> agarose embedded into a paraffin block.  I've chilled the blocks and I'm
> sectioning on a rotary microtome, at 5 microns, with a high profile
> Accuedge blade. The paraffin surrounding the agarose sections normally, but
> the agarose portion of the block causes the blade to "skip" across it
> slightly and even chip out as if my blade isn't snug in the blade holder
> (it is).  If I do get a tiny portion of agarose on my section to float out
> on the waterbath it flies away (like adding ETOH to a waterbath with
> sections already on it.)   Everyone I have asked about this says the
> agarose should section normally with the paraffin like any other FFPE
> blocks. Any ideas on why this agarose is behaving this way for me?  Thank
> you in advance!
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>