[Histonet] restaining of IHC-slides

[Colleen Forster]

Gudrun,

One you have done the HIER it is done. No need top repeat. The one thing is
that both antibodies need to have the same buffer. Fro example both citrate
or both EDTA.

Colleen Forster HT(ASCP)QIHC

On Thu, Jun 27, 2024 at 8:36 AM Gudrun Lang via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi!
>
> An already stained IHC-slide for a special antibody with HIER as
> pretreatment (eg 48 min) should be restained with another antibody.
>
> For doublestaining one has to do a second short HIER (eg 8 min) to
> denaturate the detection-antibodies before the second primary antibody.
>
> So a certain amount of HIER should be necessary before restaining. But I am
> an uncertain about the intensitiy of HIER.
>
> Is the HIER of the first staining still "active" and we need just a short
> time?
>
> Or should we perform the whole protocol for the restaining with the danger
> of tissue-damage?
>
>
>
> How do you handle this problem?
>
>
>
> Thanks in advance
>
> Gudrun Lang
>
>
>
> Linz, Austria
>
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-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930