[Histonet] restaining of IHC-slides

[Gudrun Lang]

Hi!

An already stained IHC-slide for a special antibody with HIER as
pretreatment (eg 48 min) should be restained with another antibody.

For doublestaining one has to do a second short HIER (eg 8 min) to
denaturate the detection-antibodies before the second primary antibody.

So a certain amount of HIER should be necessary before restaining. But I am
an uncertain about the intensitiy of HIER.

Is the HIER of the first staining still "active" and we need just a short
time?

Or should we perform the whole protocol for the restaining with the danger
of tissue-damage?



How do you handle this problem?



Thanks in advance

Gudrun Lang



Linz, Austria