[Histonet] cloudy cornea after Hartman's fix

[Tony Henwood]

Hartman's (also known as Davidson's) fixative is sometimes used to reveal lymph nodes in resections where they appear opaque - white. It is not surprising that the tissues of the eye would react the same.
I assume that the alcohol causes the bleaching of the tissue (or is it the acetic acid?) - more research needed.


Regards,



Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)

Principal Scientist, the Children’s Hospital at Westmead (Retired)

Adjunct Fellow, School of Medicine, University of Western Sydney.

________________________________
From: Davoli, Katherine A via Histonet <histonet at lists.utsouthwestern.edu>
Sent: 28 June 2024 04:12
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] cloudy cornea after Hartman's fix

Anyone know why the cornea of my pig eye got white/cloudy on dropping it in to Hartman's fixative?  I'm used to working with mice where, if this happens I didn't notice.

Katherine Davoli, MDiv, HTL(ASCP)cm    (they/them/theirs)
Lab Manager, Tissue Culture & Histology Cores, U. Pitt Dept of Ophthalmology
7.373 UPMC Mercy Pavilion        1622 Locust St., Pittsburgh PA 15219
(412) 624-8508   this number cannot receive texts
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet