[Histonet] cloudy cornea after Hartman's fix
Tony Henwood
afhenwood at outlook.com
Thu Jun 27 18:17:26 CDT 2024
Hartman's (also known as Davidson's) fixative is sometimes used to reveal lymph nodes in resections where they appear opaque - white. It is not surprising that the tissues of the eye would react the same.
I assume that the alcohol causes the bleaching of the tissue (or is it the acetic acid?) - more research needed.
Regards,
Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
Principal Scientist, the Children’s Hospital at Westmead (Retired)
Adjunct Fellow, School of Medicine, University of Western Sydney.
________________________________
From: Davoli, Katherine A via Histonet <histonet at lists.utsouthwestern.edu>
Sent: 28 June 2024 04:12
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] cloudy cornea after Hartman's fix
Anyone know why the cornea of my pig eye got white/cloudy on dropping it in to Hartman's fixative? I'm used to working with mice where, if this happens I didn't notice.
Katherine Davoli, MDiv, HTL(ASCP)cm (they/them/theirs)
Lab Manager, Tissue Culture & Histology Cores, U. Pitt Dept of Ophthalmology
7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219
(412) 624-8508 this number cannot receive texts
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