[Histonet] Cryotomy help

[Davoli, Katherine A]

The initial freeze isn't usually the problem.  It's what happens when it warms back up to Cryostat cutting temperatures of -20 or -25.
You'd need to get the methanol out of the tissue completely because it causes the support to melt at the temperatures the Cryostat cuts at.

And unfortunately I have not found a method that fully removes ethanol or methanol from a tissue sample that would then allow me to freeze and cut it.  If someone else replies with one I will genuinely be delighted because I have this problem a lot.

Katherine Davoli, MDiv, HTL(ASCP)cm    (they/them/theirs)
Lab Manager, Tissue Culture & Histology Cores, U. Pitt Dept of Ophthalmology
7.373 UPMC Mercy Pavilion        1622 Locust St.,Pittsburgh PA 15219
(412) 624-8508   this number cannot receive texts

-----Original Message-----
From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Friday, July 12, 2024 12:04 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cryotomy help

I have a researcher who is trying to mass spectrometry on frozen tissue sections. They submitted the sample in methanol and even after sitting in a
-80 freezer overnight the samples are too soft to section.

Does anyone have any tips on how to harden the tissue for sectioning that won't damage the results for mass spect (I was thinking liquid nitrogen but want to be sure it won't damage the results)?
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