[Histonet] Tissue falling off slides

[Rachel Gonzalez]

Hi
I used to have that problem.   A senior histologist from BioCare recommended to air dry the slides overnight and then bake them. I have not had the problem since.
Rachel

From: Ken M via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, January 31, 2024 6:07 PM
To: richard cartun <rcartun at sbcglobal.net>; histonet at lists.utsouthwestern.edu; Foshey, Annette <afoshey at mcw.edu>
Subject: Re: [Histonet] Tissue falling off slides

1) Try tap water
2) Try hydrophilic IHC slides, (I will send you samples for free)

Ken
Kdean70 at hotmail.com<mailto:Kdean70 at hotmail.com>


-----Original Message-----
From: richard cartun via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Sent: Wednesday, January 31, 2024 10:29 AM
To: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>; Foshey, Annette <afoshey at mcw.edu<mailto:afoshey at mcw.edu>>
Subject: Re: [Histonet] Tissue falling off slides

Hi Annette!
Do you put anything else in your water bath besides deionized water?
Richard CartunMorphologic Proteomics, LLC(860) 490-7633

On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:


I work with whole mount prostate blocks that I cut and stain for IHC manually. I use superfrost plus slides that I purchase from the Brain Research Laboratories. I airdry the slides after sectioning and then they are placed in a rack and dried in a 60 degree oven overnight. On some of the slides the tissue still lifts off the slides usually it is where there is tumor. Any suggestions would be greatly appreciated.

Kindest Regards,
Annette Foshey, HT
Histology Technologist, Precision Medicine Laboratory (PML)
Phone: (414) 955-7537 | E-mail: afoshey at mcw.edu<mailto:afoshey at mcw.edu<mailto:afoshey at mcw.edu%3cmailto:afoshey at mcw.edu>>
Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226

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