[Histonet] Tissue falling off slides

[Ken M]

1) Try tap water
2) Try hydrophilic IHC slides, (I will send you samples for free)

Ken
Kdean70 at hotmail.com


-----Original Message-----
From: richard cartun via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, January 31, 2024 10:29 AM
To: histonet at lists.utsouthwestern.edu; Foshey, Annette <afoshey at mcw.edu>
Subject: Re: [Histonet] Tissue falling off slides

 Hi Annette!
Do you put anything else in your water bath besides deionized water?
Richard CartunMorphologic Proteomics, LLC(860) 490-7633

    On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via Histonet <histonet at lists.utsouthwestern.edu> wrote:


I work with whole mount prostate blocks that I cut and stain for IHC manually. I use superfrost plus slides that I purchase from the Brain Research Laboratories. I airdry the slides after sectioning and then they are placed in a rack and dried in a 60 degree oven overnight.  On some of the slides the tissue still lifts off the slides usually it is where there is tumor.  Any suggestions would be greatly appreciated.

Kindest Regards,
Annette Foshey, HT
Histology Technologist, Precision Medicine Laboratory (PML)
Phone: (414) 955-7537 | E-mail: afoshey at mcw.edu<mailto:afoshey at mcw.edu>
Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet