[Histonet] tissue cassettes

[patpxs]

I'm old, old school too.  I prefer the paraffin pool myself, it helps prevent the bon-bon effect.  I had a vendor rep tell me the holding bins were designed for molten paraffin.Probably what happened over the years is that if there's too much molten wax in the bin, it over flows when the basket of cassettes is put in.Ah, displacement, just like when you get into an over full bathtub, water (or wax) everywhere. Now a days I think the dry holding tank is the rage.  As long as the tissue stays warm, it probably doesn't matter too much. Ciao bellas,PaulaSent from Samsung tablet
-------- Original message --------From: "Kurth, Virginia L via Histonet" <histonet at lists.utsouthwestern.edu> Date: 2/9/24  8:04 AM  (GMT-08:00) To: Thomas Podawiltz <tpodawiltz at yahoo.com>, "Brazie, Jeneanne E *HS" <JEB2AE at uvahealth.org>, histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] tissue cassettes I am old school and prefer them dry, lol.  I agree with Thomas, that shouldn't have that affect.Ginny-----Original Message-----From: Thomas Podawiltz via Histonet <histonet at lists.utsouthwestern.edu>Sent: Friday, February 9, 2024 8:34 AMTo: Brazie, Jeneanne E *HS <JEB2AE at uvahealth.org>; histonet at lists.utsouthwestern.eduSubject: Re: [Histonet] tissue cassettesWARNING: This email appears to have originated outside of the UW Health email system.DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe.Without seeing the blocks, that sounds more like cold molds being used, more Then, whether or not the tissues are kept in a dry, hot, well, or a wet well.Sent from Yahoo Mail for iPadOn Friday, February 9, 2024, 6:00 AM, Brazie, Jeneanne E *HS via Histonet <histonet at lists.utsouthwestern.edu> wrote:Hello :) I am encountering push back in our lab when I fill the embedding units with melted paraffin in the embedding wells. The techs here like for the tissue cassettes  to sit dry (no wax) while in the  embedding units. I find that the tissue rolls out of the sections while cutting because of a layering effect between the tissue and the paraffin its embedded in. I have communicated this but they tell me I'm "old school". Does anyone have any thoughts or opinions on this topic??_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet