[Histonet] tissue cassettes

Gudrun Lang gu.lang at gmx.at
Fri Feb 9 09:52:57 CST 2024


Hi,
Since we have turned to embedding centers in the late 80ies we let the
cassettes sit in the centers without additional paraffin.
We only see such "jumping out" tissue, when the cassettes are not warmed
(let the lid open) and the tissue renders too cold. As a result tissue and
paraffin don't combine well enough.
Maybe it is a matter of embedding technique? Too little paraffin in the mold
before setting the tissue in? Cold embedding molds? Slow handling?
Gudrun

-----Ursprüngliche Nachricht-----
Von: Brazie, Jeneanne E *HS via Histonet
[mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Freitag, 9. Februar 2024 11:41
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] tissue cassettes

Hello :) I am encountering push back in our lab when I fill the embedding
units with melted paraffin
in the embedding wells. The techs here like for the tissue cassettes  to sit
dry (no wax) while in the
 embedding units. I find that the tissue rolls out of the sections while
cutting because of a layering
effect between the tissue and the paraffin its embedded in. I have
communicated
this but they tell me I'm "old school". Does anyone have any thoughts or
opinions on this topic??

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