[Histonet] Hale vs. Alcianblue

[John Kiernan]

According to McManus & Mowry, Staining Methods (1960). page 137:
"Alcian blue is preferable to the Hale or Rinehart iron stains in that only one solution is required and direct staining occurs readily."  Their stain solution was 1% alcian blue in 4% acetic acid, pH 2.6.
Use only alcian blue powder from a batch certified (or recertified) by the Biological Stain Commission less than 5 years ago. This is one of the very few dyes that can deteriorate in the solid state. The chemical identities of dyes sold as "alcian blue" are variable; none are the original CI 74240, Ingrain blue 1. See Ali et al (2022) Biotech. Histochem. 97:11-20.
John Kiernan.
= = =
________________________________
From: Gudrun Lang via Histonet <histonet at lists.utsouthwestern.edu>
Sent: December 8, 2024 2:53 AM
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Hale vs. Alcianblue

Dear all!

Has anyone informations, if the Hale-stain (colloidal iron or Mueller-Mowry)
can be substituted by Alcianblue-stain in any application?



We only use the Hale-stain for renal cellcarcinoma for differentiation
between chromophobe and clearcell carcinoma. And I’d like to know, if this
is really necessary.



Thanks in advance

Gudrun





Gudrun Lang

Landgutstraße 25

4040 Linz



_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet