[Histonet] IHC protocols for mice and rats

[Val T]

Hi Charles-

I use:

Ki-67 from Thermo, Ref #RM-9106-S @1:100
Cleaved Caspase 3 from Cell Signaling, #9664 @1:1500

Both are rabbit-derived antibodies and both work very well on mouse, rat, human, and xenograft tissues.

Val

> On Dec 5, 2024, at 10:14 AM, Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Does anyone have a protocol for IHC testing on mice and rats for Ki-67 and
> Caspase-3 active cleaved?
> Mainly I am looking for what species primary antibody works best with which
> secondary species antibody.
> 
> I am trying to work out the background issues I am having with my detection
> kit and stainer (its not an open system) so I am looking to see where I can
> make changes to clean up my results (I can send more details in personal
> responses)
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