[Histonet] Oven Temps and baking times used for IHC's

[Cooper, Brian]

We wick our sections dry and then incubate for 15 to 20 minutes @ 70-75 degrees in a convection oven.  We tried lower temps and longer baking times for many of our markers, but we didn't see any discernable staining difference.

Thank you,

Brian Cooper, HT, QIHC (ASCP)
Histology/EM Supervisor
Children's Hospital Los Angeles
323-361-3357
bcooper at chla.usc.edu
Sent from my mobile

________________________________
From: Naira Margaryan via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, August 8, 2024 5:36:59 PM
To: Eileen Akemi Allison <akemiat3377 at gmail.com>
Cc: Histonet <Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Oven Temps and baking times used for IHC's (EXTERNAL EMAIL)

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Interesting. I never baked my slides. Left air dry o/n on room. Next day
started with deparaffinization steps 2x30min. My most AR where in the
citrate buffer in the water bath with 92C(if I remember correctly) for 37
minutes then 12 minutes cooling at room temperature. Never had a problem.
Naira

On Thu, Aug 8, 2024 at 11:28?AM Eileen Akemi Allison via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Good morning Histo Peeps!
>
> I am taking a poll on what various labs are using for temperature and
> times for IHC's slides which are baked in the oven.  It has been my
> experience while working at Biocare Medical, we would 1st wick excess water
> off the slides, then tap the rack of slides on paper; then subsequently
> place the rack of slides in a 36 degree oven for 30 minutes, then up the
> temperature to 60-62 degrees for an additional time of 30-60 minutes.
> Sorry, my brain cells can't remember which length of time in 60 degrees, I
> left Biocare Medical in 2006.  My ex, David Tacha, Ph.D, HT/HTL gave
> several workshops on this subject internationally.
>
> The UCSF Surgical Pathology lab 1st wicks excess water off, then bakes
> their IHC slides at 62 degrees for 2 hours.  I have consulted w/ some labs
> who bake their IHC slides at 76 degrees for 20 minutes.  It has been my
> experience that higher than 62 degree temps will make the Ab lose their
> antigenicity.  CK Ab's love a higher temp but most other Ab's do not.
>
> Any thoughts, information, and papers on this subject would be greatly
> appreciated.  Thank you and have a wonderful upcoming weekend!
>
> Best regards,
> Akemi
>
> Akemi Allison-Tacha BS, HT/HTL (ASCP)
> Pathology Laboratory Manager at Golden State Dermatology
> 370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598
> Cell: (408) 335-9994
> Email: aallison at gsdermca.com <mailto:aallison at gsdermca.com>
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