[Histonet] Oven Temps and baking times used for IHC's

[Naira Margaryan]

Interesting. I never baked my slides. Left air dry o/n on room. Next day
started with deparaffinization steps 2x30min. My most AR where in the
citrate buffer in the water bath with 92C(if I remember correctly) for 37
minutes then 12 minutes cooling at room temperature. Never had a problem.
Naira

On Thu, Aug 8, 2024 at 11:28 AM Eileen Akemi Allison via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Good morning Histo Peeps!
>
> I am taking a poll on what various labs are using for temperature and
> times for IHC’s slides which are baked in the oven.  It has been my
> experience while working at Biocare Medical, we would 1st wick excess water
> off the slides, then tap the rack of slides on paper; then subsequently
> place the rack of slides in a 36 degree oven for 30 minutes, then up the
> temperature to 60-62 degrees for an additional time of 30-60 minutes.
> Sorry, my brain cells can’t remember which length of time in 60 degrees, I
> left Biocare Medical in 2006.  My ex, David Tacha, Ph.D, HT/HTL gave
> several workshops on this subject internationally.
>
> The UCSF Surgical Pathology lab 1st wicks excess water off, then bakes
> their IHC slides at 62 degrees for 2 hours.  I have consulted w/ some labs
> who bake their IHC slides at 76 degrees for 20 minutes.  It has been my
> experience that higher than 62 degree temps will make the Ab lose their
> antigenicity.  CK Ab’s love a higher temp but most other Ab’s do not.
>
> Any thoughts, information, and papers on this subject would be greatly
> appreciated.  Thank you and have a wonderful upcoming weekend!
>
> Best regards,
> Akemi
>
> Akemi Allison-Tacha BS, HT/HTL (ASCP)
> Pathology Laboratory Manager at Golden State Dermatology
> 370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598
> Cell: (408) 335-9994
> Email: aallison at gsdermca.com <mailto:aallison at gsdermca.com>
> Website: www.goldenstatedermatology.com <
> http://www.goldenstatedermatology.com/>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>