[Histonet] IHC help

[Carl Hobbs]

Hi
I have same problem with Pwax Ms/rat skin, ear, knee joint sections
I always lose the bone/cartilage but......everything else is retained by using HIER at 90C for a time optimised by your lab
Sure, collagen does NOT like HIER
You know this already, I am sure
Maybe your abs will work on alternative AR?
eg: Proteinase K?
This will NOT disrupt tissue sections, provided you don't over-digest

I have posted sensitivity comparisons on Histonet images...I think....giving anti GFP as an example


Good luck
🙂


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson SPaRC
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6810