[Histonet] IHC help

[Michelle Bell]

If you’re doing online retrieval/deparaffinization, try deparafinizing offline.  I found the paraffin we loved had was higher in polymers and resulted in the tissue being prone to lifting.  As soon as we started deparafinizing BEFORE putting the slides on the instrument, we had greater (not perfect, mind you) success.
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From: Renee Fisher via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, August 1, 2024 2:39:01 PM
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] IHC help

Does anyone have a technique that will keep mice skin tissue on the slide through the retrieval process. We are using positive charged slides, but still have tissue lifting. We do digital imaging and AI the lifting of the tissue affects the AI.
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