[Histonet] Stored frozen fatty muscle tissue

[Mac Donald, Jennifer]

Are you allowing them to come up to the temperature of the cryostat before cutting?

-----Original Message-----
From: Gerard Spoelstra via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, September 26, 2023 3:45 AM
To: Histonet at lists.utsouthwestern.edu
Subject: [Histonet] Stored frozen fatty muscle tissue

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Hi Histonetters

I've been trying to cut frozen fatty muscle tissue from 2018/2019 stored at -80C. I managed to cut some sections from the same tissue on fresh frozen tissue in 2018. I suspect that storage may contribute to the difficulty to cut this. I have yet touse dry ice to further lower the temperature. They are only small biopsies, but are heavily marbled.
Any ideas?

Gerard Spoelstra

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