[Histonet] Stored frozen fatty muscle tissue

[Gerard Spoelstra]

Hi Histonetters

I’ve been trying to cut frozen fatty muscle tissue from 2018/2019 stored at -80C. I managed to cut some sections from the same tissue on fresh frozen tissue in 2018. I suspect that storage may contribute to the difficulty to cut this. I have yet touse dry ice to further lower the temperature. They are only small biopsies, but are heavily marbled.
Any ideas?

Gerard Spoelstra