[Histonet] Histonet Digest, Vol 232, Issue 7

[Eddie Martin]

Hi KDean70 at hotmail.com,

What likely is occurring but you didn’t mention in the thread is that your
friend is using alkaline phosphatase based detection, which, by itself can
react with kidney tissue. This likely explains why your friend is getting
strong kidney staining and weak melan-a staining.

This also tells me your friend didn’t optimize the antibody correctly. I’m
not sure why your friend is choosing to use a lyophilized antibody for
Melan-A as so many other commercially available options are available
capable of refrigerated storage and good up to 3 years. CellMarque makes an
Melan-A in both realty to use and/or a liquid concentrate with a 3-year
expiry date.

Best wishes,
Eddie Martin, HTL,QIHC
The National Institutes of Health
Department of Laboratory Medicine
Bone Marrow Service
Eddie.martin at nih.gov
(301)-594-2054



On Wed, Mar 15, 2023 at 1:00 PM <histonet-request at lists.utsouthwestern.edu>
wrote:

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>    1. Problems with Melan A staining (Ken M)
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> ---------- Forwarded message ----------
> From: Ken M <kdean70 at hotmail.com>
> To: "histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu
> >
> Cc:
> Bcc:
> Date: Wed, 15 Mar 2023 16:51:03 +0000
> Subject: [Histonet] Problems with Melan A staining
> One of my histotech friends is having issues with Melan A staining. Using
> MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue
> control was stained strongly positive while the positive melanoma tissue
> control was stained weakly positive. He couldn’t figure out why after
> several tries to reduce background. Can someone give me some suggestions?
> Thank you very much!
>
>
> Ken
>
>
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