[Histonet] IHC gunk

[Mac Donald, Jennifer]

Is it possible that it is hematoxylin precipitate?

-----Original Message-----
From: Karen Heckford CA-San Francisco via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Wednesday, March 22, 2023 10:45 AM
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] IHC gunk

  EXTERNAL SENDER - Exercise caution with requests, links, and attachments.

Good Morning,
I have recently developed a problem with contamination of some kind on my IHC slides.
The contamination is black clumps and lays on top of the tissue.  I have
been told it is bacteria but the Pathologist and I kind of doubt that.   I
cannot get a good picture of it to show.  It may not be on all the slides on the same run or even the same antibody slide.

I have decontaminated the whole system.  I have put fresh reagents on the
instrument.   This stuff looks like it would wash off at the end of the run
since it is sitting right on top of the tissue.  I do not see this stuff
elsewhere on the slide, only the tissue.   Does not matter if the tissue
was cut fresh or not.

I have tried everything I can think of to get rid of it and still have this issue.  I use DiH20 from the hospital system.  Not sure if this may be the problem.  I can have Engineering test the DiH20.

Any help would be greatly appreciated.

Thanks,

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-750-5751

karen.heckford@ <karen.heckford at dignityhealth.org>commonspirit.org




Caution:  This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.  Any further review, dissemination, distribution, or copying of this message is strictly prohibited.  If you have received this communication in error, please notify us  immediately by reply email.  Thank you

Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cjmacdonald%40mtsac.edu%7C27946f7c47f74e58436a08db2afd6592%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C638151040067352929%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=coq7k8kIhG03oke%2B477suyaDCCby1zEujMseWZ4lQSw%3D&reserved=0