[Histonet] IHC staining of tendons and cartilage

[Shirley A. Powell]

Hi Charles, 

Shirley Powell here in humid Georgia.  I ran an IHC reference lab here for many years.  I had a problem with using charged slides for a lot of the tissues I processed.  I used manual and automation methods.  My tissues were washing off a lot.  I changed to an adhesive for the water bath called Sta-On and I think Surgipath was the company that made it.  Surgipath was bought out by Leica but they still sold it.  Sta-On was the best adhesive I had found and that worked for me for many years.  Whenever I do IHC that is what I use, especially bone, cartilage, bloody specimens, autopsy tissues, they stay on better.  Some other companies may be selling it now, like VWR/Avantor.  Just Google it. 
 
Shirley

Shirley Powell, HTL(ASCP)
Technical Director Histology Curricular Support Laboratory
Pathology Department
Mercer University School of Medicine
powell_sa at mercer.edu
Phone: 478-301-2374
https://medicine.mercer.edu/

-----Original Message-----
From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Tuesday, March 21, 2023 2:31 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IHC staining of tendons and cartilage

Hello all,

I am in a new position and it will potentially require doing a lot of IHC testing on cartilage and tendon samples. I have done some practice runs on my automated stainer and manually and am running into issues with the tissue sections falling off completely or folding over on itself during each process.

If anyone does staining like this routinely and has some pointers/tricks to try to get the samples to adhere to the slides better it would be greatly appreciated.

I have tried using charged slides from a variety of vendors and get similar results across the board.
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