[Histonet] IHC staining of tendons and cartilage

Charles Riley criley at udel.edu
Tue Mar 21 13:30:34 CDT 2023


Hello all,

I am in a new position and it will potentially require doing a lot of IHC
testing on cartilage and tendon samples. I have done some practice runs on
my automated stainer and manually and am running into issues with the
tissue sections falling off completely or folding over on itself during
each process.

If anyone does staining like this routinely and has some pointers/tricks to
try to get the samples to adhere to the slides better it would be greatly
appreciated.

I have tried using charged slides from a variety of vendors and get similar
results across the board.


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