[Histonet] background staining Melan A

[Terri Braud]

My first instinct is to ask what detection system you are using.  If you are using an Avidin-Biotin detection, kidney is notorious for having excess biotin that must be blocked, or better yet, use a polymer based detection system.
That needs to be answered before further trouble shooting.

Terri L. Braud, HT(ASCP)
HNL Laboratories for 
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-2021
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Today's Topics:

   1. Problems with Melan A staining (Ken M)
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Message: 1
Date: Wed, 15 Mar 2023 16:51:03 +0000
From: Ken M <kdean70 at hotmail.com>
Subject: [Histonet] Problems with Melan A staining
One of my histotech friends is having issues with Melan A staining. Using MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue control was stained strongly positive while the positive melanoma tissue control was stained weakly positive. He couldn?t figure out why after several tries to reduce background. Can someone give me some suggestions? Thank you very much!
Ken