[Histonet] Mice/Rat brain

[Paula Sicurello]

Hi Renee, 
Brains are notorious for being difficult.  Processing has to be just so, embedding has to be just so, and sectioning?  Well, just plain tricky.
It sounds like your water bath may be a smidge too cool.  Brains are picky that way.  Water bath too warm?  Poof!  The section explodes.  Too cool? Wrinkles.  
Can you use vibratome sections or do you need FFPE sections?

Paula Sicurello 
 
  On Thu, Mar 9, 2023 at 6:51 AM, Renee Fisher via Histonet<histonet at lists.utsouthwestern.edu> wrote:   
We are having issues with mice and rat brain sections. There are folds in the tissue. We just can’t seem to get a flat section without folds. Any suggestions?
Sent from my iPhone
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