[Histonet] destain for IHC

[Tony Reilly]

Hi

If you are using the peroxidase method the hydrogen peroxide step will remove the eosin and some of the haematoxylin.

No need to do a decolorisation step before staining.

As Gudren said the only problem may be not having coated slides.

Good luck.
Sadly retired histologist
Tony

Sent from my iPhone

> On 10 Feb 2023, at 6:07 am, Katleba, Maria via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Oh no!!! We cut all slides on + 
> 
> BUT you can do this. It’s what we do when we have H&E on plain slides.
> 
> Whether  you have a dry unstain from another lab that sent u a slide, or your H&E that you just took the coverslip off…
> 
> Place it in. Coplin jar with 1-3 DROPS of formalin, cover, place In 60ft degree oven for 60 min. You want the bottom of the jar to be moist not floating in it 
> 
> After 60 min remove and open UNDER A HOOD (it’s a vaporized carcinogen)
> Air out 10-15 min… place on stainer 
> 
> The drops vaporize and the aldehyde goes through tissue and make it adhere to the slide permanently.
> 
> I do this when I cut nails or anything that won’t stay on a plus slide.
> 
> Maria
> 
> Sent from my iPhone
> 
>> On Feb 8, 2023, at 11:21 PM, Gudrun Lang via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>> 
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