[Histonet] Sudan Black B

[John Kiernan]

The Sudan black B method for lipofuscin is exactly the same as for frozen sections, but it is applied to hydrated paraffin sections. Remember that the dye solution needs to be fairly fresh (less than 4 weeks old) and must be filtered immediately before using. Use Sudan black B (CI 26150) powder that has been certified by the Biological Stain Commission.

Various controls and other stains are needed to be certain that the pigment is lipofuscin, not melanin or haemosiderin.

John Kiernan
Anatomy, UWO, London, Canada
= = =
________________________________
From: Betsy Molinari via Histonet <histonet at lists.utsouthwestern.edu>
Sent: August 10, 2023 10:57 AM
To: Histonet <Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Sudan Black B

Hi,
I have been asked to do a Sudan stain on a heart  biopsy for lipofuscin. The biopsy is in a paraffin block. They are looking  to better report and understand the IHC. I am totally unfamiliar with this stain. I did some reading but have been unable to find a protocol for paraffin sections. I found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't have that edition.  Any ideas would be greatly appreciated .

Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
1101 Bates St.
Houston, Texas  77030
832-355-6524

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

The Texas Heart Institute (r)
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org
texasheart.org<https://www.texasheart.org/> | texasheartmedical.org<https://www.texasheartmedical.org/> | facebook<https://www.facebook.com/Texas.Heart.Institute> | twitter<https://twitter.com/Texas_Heart>

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________________________________
From: Betsy Molinari via Histonet <histonet at lists.utsouthwestern.edu>
Sent: August 10, 2023 10:57 AM
To: Histonet <Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Sudan Black B

Hi,
I have been asked to do a Sudan stain on a heart  biopsy for lipofuscin. The biopsy is in a paraffin block. They are looking  to better report and understand the IHC. I am totally unfamiliar with this stain. I did some reading but have been unable to find a protocol for paraffin sections. I found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't have that edition.  Any ideas would be greatly appreciated .

Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
1101 Bates St.
Houston, Texas  77030
832-355-6524

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

The Texas Heart Institute (r)
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org
texasheart.org<https://www.texasheart.org/> | texasheartmedical.org<https://www.texasheartmedical.org/> | facebook<https://www.facebook.com/Texas.Heart.Institute> | twitter<https://twitter.com/Texas_Heart>

CONFIDENTIALITY NOTICE: This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet