[Histonet] Sudan Black B

[Betsy Molinari]

Hi,
I have been asked to do a Sudan stain on a heart  biopsy for lipofuscin. The biopsy is in a paraffin block. They are looking  to better report and understand the IHC. I am totally unfamiliar with this stain. I did some reading but have been unable to find a protocol for paraffin sections. I found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't have that edition.  Any ideas would be greatly appreciated .

Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
1101 Bates St.
Houston, Texas  77030
832-355-6524

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

The Texas Heart Institute (r)
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org
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