[Histonet] maximum thickness brain in slide

[Carl Hobbs]

Hi Alonso
Firstly, what antibody( ies) are you wanting to use on your Pwax sections?
If you are looking just to identify proteins,  8 microns is sufficient
I mount my brain sections ( human, ms, rat, axolotl, worm, fruitfly, pig...etc) then airdry them O/N in a working fume hood
Then, before dewaxing, I put them into a 60C oven for 1hr.

Imho, free-floating sections are useful only if you wish to study projections over depth

Use Superfrost Plus ( or equivilent) slides

Good luck!
Carl


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6810