[Histonet] Reconstituting antibodies and dilutions

[Gudrun Lang]

500 µg in 1000µl  is the same as 100µg in 200µl

So I would reconstitute the 100 µg with 200 µl of water to get the liquid
antibody-concentrate.
Then I would make the working-dilution: eg. 5µl of concentrate + 5000µl
buffer 

The correct way would be 5 µl + 4995 µl, but in practice this does'nt
matter.

Gudrun Lang

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Von: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Donnerstag, 13. Oktober 2022 17:49
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] Reconstituting antibodies and dilutions

When calculating the dilution of an antibody when do start?

i.e.  If i have 100 ug of lyophilized antibody and its recommended
reconstitution is 500 ug/ml

How do I mix a 1:1000 dilution?

I am thinking it's adding 2mL diH20 to create the 500 ug/mL and
then diluting the sample.
And I would be able to make 2000ml of 1:1000 concentration solution ?  2mL
antibody and 1998mL diluent?


Please let me know if I made a calculation error
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