[Histonet] Reconstituting antibodies and dilutions
Gudrun Lang
gu.lang at gmx.at
Thu Oct 13 13:22:41 CDT 2022
500 µg in 1000µl is the same as 100µg in 200µl
So I would reconstitute the 100 µg with 200 µl of water to get the liquid
antibody-concentrate.
Then I would make the working-dilution: eg. 5µl of concentrate + 5000µl
buffer
The correct way would be 5 µl + 4995 µl, but in practice this does'nt
matter.
Gudrun Lang
-----Ursprüngliche Nachricht-----
Von: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Gesendet: Donnerstag, 13. Oktober 2022 17:49
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] Reconstituting antibodies and dilutions
When calculating the dilution of an antibody when do start?
i.e. If i have 100 ug of lyophilized antibody and its recommended
reconstitution is 500 ug/ml
How do I mix a 1:1000 dilution?
I am thinking it's adding 2mL diH20 to create the 500 ug/mL and
then diluting the sample.
And I would be able to make 2000ml of 1:1000 concentration solution ? 2mL
antibody and 1998mL diluent?
Please let me know if I made a calculation error
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
More information about the Histonet
mailing list