[Histonet] Reconstituting antibodies and dilutions

[Charles Riley]

When calculating the dilution of an antibody when do start?

i.e.  If i have 100 ug of lyophilized antibody and its recommended
reconstitution is 500 ug/ml

How do I mix a 1:1000 dilution?

I am thinking it's adding 2mL diH20 to create the 500 ug/mL and
then diluting the sample.
And I would be able to make 2000ml of 1:1000 concentration solution ?  2mL
antibody and 1998mL diluent?


Please let me know if I made a calculation error