[Histonet] Reconstituting antibodies and dilutions
Charles Riley
criley at udel.edu
Thu Oct 13 10:49:26 CDT 2022
When calculating the dilution of an antibody when do start?
i.e. If i have 100 ug of lyophilized antibody and its recommended
reconstitution is 500 ug/ml
How do I mix a 1:1000 dilution?
I am thinking it's adding 2mL diH20 to create the 500 ug/mL and
then diluting the sample.
And I would be able to make 2000ml of 1:1000 concentration solution ? 2mL
antibody and 1998mL diluent?
Please let me know if I made a calculation error
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