[Histonet] Manual double staining IHC

Fri Nov 18 12:05:19 CST 2022

Hi Charles, protocols are going to depend on the species of your primary Abs you're looking to combine.  If it's mouse and rabbit you can stain simultaneously.  I use Biocare's double stain 1 or double stain 2 polymer depending on which marker you want on what colour.

If your Abs are the same species then you may need to stain sequentially with a denaturation step in between.  

Feel free to shoot me an email with the Abs you're using and I can help with some suggestions.

Katy Milne
MCIC-Histology, Deeley Research Centre
BC Cancer Victoria

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Today's Topics:

   1. Re: Testing Skills (Terri  Braud)
   2. Manual double staining IHC (Charles Riley)
   3. Histotechnician and Medical Technologist positions - Fred
      Hutchinson - Seattle (Donato, Elizabeth)
   4. Re: Manual double staining IHC (Frazer Bell)

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Message: 1
Date: Thu, 17 Nov 2022 18:16:51 +0000
From: "Terri  Braud" <tbraud at holyredeemer.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Testing Skills
Message-ID: <ef1beb923cd442c6b0435151aabfe746 at holyredeemer.com>
Content-Type: text/plain; charset="us-ascii"

Aside from the terrible legal  liability that you would open your institution to by asking them to operate "dangerous" equipment when they are not in your employ, sitting at a strange microtome/waterbath set up with different knives, paraffins, processing, just would not be an accurate test.
I really liked Thomas Podawiltz's idea.  Usually I just ask leading questions and then evaluate during the probation period.

Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874

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Message: 2
Date: Thu, 17 Nov 2022 14:25:50 -0500
From: Charles Riley <criley at udel.edu>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Manual double staining IHC
Message-ID:
        <CAHMJNChEW21NJMPoZuEFOP69n0fK0su=trUk-K12F6rzVP35ZQ at mail.gmail.com>
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Hello all,

I have never done double staining manually and wanted to know if anyone has protocols or suggestions for doing this?

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Message: 3
Date: Thu, 17 Nov 2022 20:03:27 +0000
From: "Donato, Elizabeth" <edonato at fredhutch.org>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Histotechnician and Medical Technologist positions
        - Fred Hutchinson - Seattle
Message-ID:
        <MW5PR11MB580916C4707AC1A800802FBAA3069 at MW5PR11MB5809.namprd11.prod.outlook.com>

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Hello Everyone,

I hope you will consider sharing these job postings with your colleagues.  Fred Hutchinson Cancer Center is a wonderful organization to work for and the Clinical Trials Pathology lab in particular is involved in impressive, cutting-edge research.

Thank you,

Liz

Research Histotechnician I/II

https://careers-fhcrc.icims.com/jobs/23684/research-histotechnician-i-ii/job

Clinical Research Technologist II

https://careers-fhcrc.icims.com/jobs/23780/clinical-research-technologist-ii/job

www.fredhutch.org<http://www.fredhutch.org/>

Liz Donato

Manager | Specialized Pathology Laboratory

Clinical Research Division

Fred Hutchinson Cancer Center

?????????????????????????????????????????????????????????????????????????????????????????????

206.667.4501 p | 425.443.6388 c | 206.667.5255 f | edonato at FredHutch.org<mailto:edonato at FredHutch.org>

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Message: 4
Date: Fri, 18 Nov 2022 07:47:21 +0000
From: Frazer Bell <Frazer.Bell at glasgow.ac.uk>
To: Charles Riley <criley at udel.edu>,
        "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Manual double staining IHC
Message-ID:
        <LNXP265MB0458D7517DD61373AC8A22BBB1099 at LNXP265MB0458.GBRP265.PROD.OUTLOOK.COM>

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Hi,

We have successfully done double staining using both DAB and Magenta on our Dako autostainer, you could do it manually.

If you would like further information please let me know.

Frazer Bell
Histopathology Technician

Histology Research Service/ Veterinary Diagnostics Services
307 Jarrett Building
School of Biodiversity, One Health & Veterinary Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Bearsden Road Glasgow
G61 1QH
Tel: +44 (0) 141 330 6933
email: vet-histology at glasgow.ac.uk
Website: http://www.gla.ac.uk/histology
The University of Glasgow, charity number SC004401

-----Original Message-----
From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu>
Sent: 17 November 2022 19:26
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Manual double staining IHC

Hello all,

I have never done double staining manually and wanted to know if anyone has protocols or suggestions for doing this?
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