[Histonet] Super cold cryostat cutting head temperatures

[Caroline Miller]

I have used down to -30 a few times for research samples, mainly to try and
get stuff to cut better rather than that is what was requested. Everything
gets very brittle at that temperature, including your hands after a short
while.

Never -60 though!

Maybe that is just for the peltier freezing mechanism? For putting the
tissue on the chucks with OCT glue?

I typically will set the specimen head a little lower than the chamber. But
only 3-4 degrees

This is the one I typically use and it is -40 max:
https://www.leicabiosystems.com/us/histology-equipment/cryostats/leica-cm3050-s/

I would be interested to know if anyone works at -60 in the LM world!

Caroline


On Thu, May 19, 2022 at 12:37 PM Colleen Forster via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Thank you Paul.
>
> Colleen
>
> On Thursday, May 19, 2022, P Sicurello <patpxs at gmail.com> wrote:
>
> > Cryo-electron microscopy cuts thin sections at temperatures colder than
> > that.  It is used to preserve antigenicity in certain situations.
> >
> > However, a cryostat is not used.  An ultramicrotome fitted with specific
> > cryo attachments is used.
> >
> > Sincerely,
> >
> > Paula Sicurello
> >
> > Sent from my iPhone
> >
> > > On May 18, 2022, at 12:55 PM, Colleen Forster via Histonet <
> > histonet at lists.utsouthwestern.edu> wrote:
> > >
> > > Following....I am in research and I have never cut this cold.
> > >
> > > Colleen Forster
> > >
> > >> On Wed, May 18, 2022 at 2:04 PM Ken Marzinsky via Histonet <
> > >> histonet at lists.utsouthwestern.edu> wrote:
> > >>
> > >> Can anyone tell me why research labs often require cryostat cutting
> head
> > >> temperatures down to -60C?
> > >>
> > >> Ken
> > >> Durham, NC
> > >> _______________________________________________
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> > >>
> > >
> > >
> > > --
> > > Colleen Forster HT(ASCP)QIHC
> > > BLS Histology and IHC Laboratory
> > > Jackson Hall, Room 2-155
> > > 321 Church St. SE
> > > Minneapolis, MN 55455
> > > 612-626-1930
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> > > Histonet at lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> --
> Colleen Forster HT(ASCP)QIHC
> BLS Histology and IHC Laboratory
> Jackson Hall, Room 2-155
> 321 Church St. SE
> Minneapolis, MN 55455
> 612-626-1930
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
-- 
Caroline Miller, M.Sc
Histology, Imaging, and Image analysis consultant
415 2187297