[Histonet] Super cold cryostat cutting head temperatures

[Colleen Forster]

Thank you Paul.

Colleen

On Thursday, May 19, 2022, P Sicurello <patpxs at gmail.com> wrote:

> Cryo-electron microscopy cuts thin sections at temperatures colder than
> that.  It is used to preserve antigenicity in certain situations.
>
> However, a cryostat is not used.  An ultramicrotome fitted with specific
> cryo attachments is used.
>
> Sincerely,
>
> Paula Sicurello
>
> Sent from my iPhone
>
> > On May 18, 2022, at 12:55 PM, Colleen Forster via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
> >
> > Following....I am in research and I have never cut this cold.
> >
> > Colleen Forster
> >
> >> On Wed, May 18, 2022 at 2:04 PM Ken Marzinsky via Histonet <
> >> histonet at lists.utsouthwestern.edu> wrote:
> >>
> >> Can anyone tell me why research labs often require cryostat cutting head
> >> temperatures down to -60C?
> >>
> >> Ken
> >> Durham, NC
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> >>
> >
> >
> > --
> > Colleen Forster HT(ASCP)QIHC
> > BLS Histology and IHC Laboratory
> > Jackson Hall, Room 2-155
> > 321 Church St. SE
> > Minneapolis, MN 55455
> > 612-626-1930
> > _______________________________________________
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930