[Histonet] Active Caspase-3 IHC-P staining

[Val Tolley]

If you have access to tissues, rather than purchasing cell lines, you can use spleen, gut, lymph nodes.  They will all have a lot of apoptosis. You can use a lot of different tissues for negative controls: I prefer brain or muscle since they are less dense with nuclei.

Val

> On Jun 23, 2022, at 10:32 AM, Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> I am currently running a research project and the researcher wants me to
> use Caspase-3 for apoptosis staining. I have never worked with this
> antibody before and want to know if anyone has any suggestions on what to
> use for a positive tissue control and a negative tissue control?
> 
> 
> I purchased the antibody from Novus Biologicals and they recommend a Jurkat
> cell lysate for positive control but I am not familiar with this as well.
> 
> Any guidance would be greatly appreciated
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