[Histonet] Active Caspase-3 IHC-P staining

[Porter, Amy]

What species are you staining??

Amy S. Porter, HT (ASCP) - Lab Supervisor
Michigan State University
Departments of Physiology & Pathology
Investigative HistoPathology Lab
567 Wilson Road - Rm 2133
East Lansing, MI  48824
Ph:  517-884-5026
(she/her/hers)
https://sites.google.com/msu.edu/ihpl/home
"Life is like riding a bicycle. To keep your balance, you must keep moving." - Albert Einstein


-----Original Message-----
From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Thursday, June 23, 2022 1:19 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Active Caspase-3 IHC-P staining

I am currently running a research project and the researcher wants me to use Caspase-3 for apoptosis staining. I have never worked with this antibody before and want to know if anyone has any suggestions on what to use for a positive tissue control and a negative tissue control?


I purchased the antibody from Novus Biologicals and they recommend a Jurkat cell lysate for positive control but I am not familiar with this as well.

Any guidance would be greatly appreciated _______________________________________________
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