[Histonet] microtome blades and wax

[Rathborne, Toni]

Jessica,

What has Leica said about the problem? I have always found them to be responsive and able to provide suggestions. This would be especially true with a new instrument, still under warranty. We have never had any inconsistencies with our Autocut, which was purchased last spring.

Toni


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-----Original Message-----
From: Piche, Jessica via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Friday, January 14, 2022 8:43 AM
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] microtome blades and wax


*** This is an External Email ***

Good Morning,

We have been using Leica Histocore Autocut microtomes for a few months and have been having inconsistency with cutting on them. We have tried so many different blades, high and low, and have tried changing the blade angle. Nothing is a perfect fix, and we feel like we are really having to work much harder to get a great section. We were wondering what everyone else uses. I was also wondering if the wax could be an issue. We use EM-400 for embedding. I'd appreciate anyone's thoughts.

Thank you and have a nice weekend!

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
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