[Histonet] Tiny folds in colon bx

[Hobbs, Carl]

Imho....your waterbath is not hot enough
The sections are not expanding
Sure, if you are floating sections directly onto water bath....baaad
Place your ribbon on a slide that has 20% alcohol on it
Float that onto your waterbath
The alcohol teases the sections nicely so....no creases.
NB: Leave in the waterbath until you see no creases
Works for me every time
NB: I do many species of gut and have no problems if.....I do the above

Good luck

Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6810