[Histonet] Automated ihc staining of bone

[Cooper, Brian]

Charles, 

Try and blot your sections dry before placing them into the oven.  After picking up your section, place your slide onto a flat surface and take a paper towel or piece of filter paper, and gently press it down on top of the section to wick away all of the moisture.  We use this technique frequently here on bone marrow cores.

Thanks, 

Brian D. Cooper, HT (ASCP)CM QIHC| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcooper at chla.usc.edu 

-----Original Message-----
From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Tuesday, August 30, 2022 12:50 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Automated ihc staining of bone (EXTERNAL EMAIL)

****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu.

I am working on trying to get IHC stains optimized on bone samples of rat tibias. The issue I am running into is that the periosteum is separating from the bone marrow and wrinkling up over top of the rest of the sample.

If anyone has any techniques to prevent this wrinkling/detachment of tissue from the main section during automated ihc staining it would be greatly appreciated.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://secure-web.cisco.com/1srYnJjJBwOg5qJEJ_epFgbAXCjZWoirSFghf1tBQDTt4Kt86glQhWXDUFKHHe2BviKDOGHc_YOZrH3k3sKiGazjO1fRhU2GFJi18RGa2bb0f-VI2JMFwgoRCh3Mv4BFJnHqPjO4A8wLuoBUVteRZSMMDezrs0-eKxExtxe_Bn1OanJnxSFmzWIQXJl9XzUCwT1uCqSj9CFXMwW4swVLbFy3qpaq_74-LnLDO6yiQlBPyYP1upb0iwltU9iWQo-lfrXjFfKlYAHruMGUbJftFh1zBiA4sOKi90ubPDFxSvFr3-Aerb35g3yHJKIG5lZ2K0RuznvEbH-EjWmb7n3uhym4J61BoaaMBF1sH4MG7XG7yiiK5CIqm9SvxKGHyM5MeQXiTb_Ehnc74qW2NkSkEur2f06DKlv742nj6i9Ge70vNqu9tXdlIft0Q4nj4mBsO/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet

CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.