[Histonet] Automated ihc staining of bone

Charles Riley criley at udel.edu
Tue Aug 30 14:50:18 CDT 2022


I am working on trying to get IHC stains optimized on bone samples of rat
tibias. The issue I am running into is that the periosteum is
separating from the bone marrow and wrinkling up over top of the rest of
the sample.

If anyone has any techniques to prevent this wrinkling/detachment of tissue
from the main section during automated ihc staining it would be greatly
appreciated.


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