[Histonet] Reprocessing Protocol

Etheridge, Sandra AFF:EX Sandra.Etheridge at gov.bc.ca
Tue Oct 12 16:19:10 CDT 2021


Hi Curt,

We have used Taggart's Method quite successfully in the past for poorly processed tissues.  You can find it online.  The isotonic saline may help to rehydrate your tissues.

1.  Melt down the tissue block in the embedding centre block tray area and gently blot off the excess wax. Place the tissue in a newly labelled cassette.
2.  Place the cassette into a beaker of isotonic saline (0.9% sodium chloride) and place it in the 65 C incubator/oven for one hour. This will melt the residual wax which will rise to the surface of the saline.
3.  Remove the cassette from the saline, drain briefly and place in your processor from formalin, using a schedule that would have been of appropriate length as used initially.
4.  Embed and section as per usual.

Good luck!

Sandra Etheridge

-----Original Message-----
From: histonet-request at lists.utsouthwestern.edu <histonet-request at lists.utsouthwestern.edu> 
Sent: October 12, 2021 10:00 AM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 215, Issue 8

[EXTERNAL] This email came from an external source. Only open attachments or links that you are expecting from a known sender.


Send Histonet mailing list submissions to
        histonet at lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
        https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Csandra.etheridge%40gov.bc.ca%7Cbdb6ae0c78de43df498d08d98da1c4da%7C6fdb52003d0d4a8ab036d3685e359adc%7C0%7C0%7C637696548191633375%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=BrEkw92hcrZCE2E7LfNRrM3Zi%2BIxWyNUSevhTPcXqdQ%3D&reserved=0
or, via email, send a message with subject or body 'help' to
        histonet-request at lists.utsouthwestern.edu

You can reach the person managing the list at
        histonet-owner at lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."


Today's Topics:

   1. reprocessing tissue (Curt Tague)


----------------------------------------------------------------------

Message: 1
Date: Tue, 12 Oct 2021 15:14:54 +0000
From: Curt Tague <c.tague at pathologyarts.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] reprocessing tissue
Message-ID:
        <SJ0PR14MB43152C53B72BE198D958A018F1B69 at SJ0PR14MB4315.namprd14.prod.outlook.com>

Content-Type: text/plain; charset="us-ascii"

I have a problem... some tissue got processed very poorly, there was water in the system somewhere and a few blocks just look burnt.. the nuclei are faint and cloudy, no detail at all. I've tried the process of rehydrating with the 30% formaldehyde, glycerol and sodium acetate solution but they still process poorly, come out very brittle and just don't look good under the scope.

Does anyone have a magic bullet to salvage these specimens? I can send a pic directly if it helps.

Thanks,
Curt



------------------------------

Subject: Digest Footer

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Csandra.etheridge%40gov.bc.ca%7Cbdb6ae0c78de43df498d08d98da1c4da%7C6fdb52003d0d4a8ab036d3685e359adc%7C0%7C0%7C637696548191633375%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=BrEkw92hcrZCE2E7LfNRrM3Zi%2BIxWyNUSevhTPcXqdQ%3D&reserved=0

------------------------------

End of Histonet Digest, Vol 215, Issue 8
****************************************



More information about the Histonet mailing list