[Histonet] Reprocessing Protocol

[Etheridge, Sandra AFF:EX]

Hi Curt,

We have used Taggart's Method quite successfully in the past for poorly processed tissues.  You can find it online.  The isotonic saline may help to rehydrate your tissues.

1.  Melt down the tissue block in the embedding centre block tray area and gently blot off the excess wax. Place the tissue in a newly labelled cassette.
2.  Place the cassette into a beaker of isotonic saline (0.9% sodium chloride) and place it in the 65 C incubator/oven for one hour. This will melt the residual wax which will rise to the surface of the saline.
3.  Remove the cassette from the saline, drain briefly and place in your processor from formalin, using a schedule that would have been of appropriate length as used initially.
4.  Embed and section as per usual.

Good luck!

Sandra Etheridge

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Today's Topics:

   1. reprocessing tissue (Curt Tague)


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Message: 1
Date: Tue, 12 Oct 2021 15:14:54 +0000
From: Curt Tague <c.tague at pathologyarts.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] reprocessing tissue
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        <SJ0PR14MB43152C53B72BE198D958A018F1B69 at SJ0PR14MB4315.namprd14.prod.outlook.com>

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I have a problem... some tissue got processed very poorly, there was water in the system somewhere and a few blocks just look burnt.. the nuclei are faint and cloudy, no detail at all. I've tried the process of rehydrating with the 30% formaldehyde, glycerol and sodium acetate solution but they still process poorly, come out very brittle and just don't look good under the scope.

Does anyone have a magic bullet to salvage these specimens? I can send a pic directly if it helps.

Thanks,
Curt



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