[Histonet] reprocessing tissue

De Guzman, Jose V Jose.R.deGuzman at gunet.georgetown.edu
Tue Oct 12 13:48:42 CDT 2021

Are these FFPE tissue? We usually just do the program in reverse order by deparaffinizing, 100% alc., 95% alc., 70% alc., water, formalin. 


-----Original Message-----
From: Curt Tague <c.tague at pathologyarts.com> 
Sent: Tuesday, October 12, 2021 11:15 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] reprocessing tissue

I have a problem... some tissue got processed very poorly, there was water in the system somewhere and a few blocks just look burnt.. the nuclei are faint and cloudy, no detail at all. I've tried the process of rehydrating with the 30% formaldehyde, glycerol and sodium acetate solution but they still process poorly, come out very brittle and just don't look good under the scope.

Does anyone have a magic bullet to salvage these specimens? I can send a pic directly if it helps.


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