[Histonet] Fixative in diff-quick

Bryan Llewellyn llewllew at shaw.ca
Mon Oct 4 16:40:53 CDT 2021

Diff Quick appears to be a modified Field's stain. The blue dye in the 
methanol is one of the modifications. Field's stain gives much the same 
staining, so using plain methanol should be of no concern. The simplest 
way to find out is surely to try it and see.


Bryan Llewellyn

Corbin, Clay via Histonet wrote:
> Hey folks,
> I am shopping for a diff-quick kit.  However, all I really need is the fixative.  Generally, there is a blue stain (triarylmethane) added to the methanol in the fixative solution.  I have a giant jug of lab grade methanol.  What would I lose by using methanol alone compared to the fixative solution included in a diff-quick kit?
> Thanks!
> Clay
> Clay Corbin, PhD
> Professor of Biology
> Bloomsburg University
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