[Histonet] Fixative in diff-quick
Bryan Llewellyn
llewllew at shaw.ca
Mon Oct 4 16:40:53 CDT 2021
Diff Quick appears to be a modified Field's stain. The blue dye in the
methanol is one of the modifications. Field's stain gives much the same
staining, so using plain methanol should be of no concern. The simplest
way to find out is surely to try it and see.
http://stainsfile.info/stain/micro/field.htm
Bryan Llewellyn
Corbin, Clay via Histonet wrote:
> Hey folks,
> I am shopping for a diff-quick kit. However, all I really need is the fixative. Generally, there is a blue stain (triarylmethane) added to the methanol in the fixative solution. I have a giant jug of lab grade methanol. What would I lose by using methanol alone compared to the fixative solution included in a diff-quick kit?
> Thanks!
> Clay
>
> Clay Corbin, PhD
> Professor of Biology
> Bloomsburg University
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
More information about the Histonet
mailing list