[Histonet] Microwave slide drying

[Cartun, Richard]

I have never used microwave drying for IHC slides.  I worry that if there is water trapped underneath the tissue section it might cause destruction of the tissue section if it is rapidly heated and "pops".  You could run a "side-by-side" comparison and see if you notice a difference.  Also, how would you monitor the temperature of the slide?  However, my philosophy has always been if it makes the process more efficient and/or less costly without changing the outcome, "do it".

Happy Thanksgiving to all who celebrate it.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD
  Immunopathology/Morphologic Proteomics Laboratory
Assistant Director, Anatomic Pathology
Department of Pathology & Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 (Fax)

-----Original Message-----
From: Samantha Golden via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, November 23, 2021 11:24 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Microwave slide drying

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What are the Histonets thoughts toward drying your slides in the microwave….. pros/cons

I would specifically like feedback regarding the slides being used for IHC.

My new lab does this and it’s new to me.

Thanks!!
Samantha
Sent from my iPhone

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