[Histonet] IF with permanent mounting media?

[Morken, Timothy]

Thanks John! This looks interesting to say the least.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

From: John Kiernan <jkiernan at uwo.ca>
Sent: Friday, May 28, 2021 3:20 PM
To: Histonet <histonet at lists.utsouthwestern.edu>; Morken, Timothy <Timothy.Morken at ucsf.edu>
Subject: Re: IF with permanent mounting media?

Espada J, Juarranz A, Galaz S, Canete M, Villanueva A, Pacheco M, Stockert JC (2005) Non-aqueous permanent mounting for immunofluorescence microscopy. Histochem. Cell Biol. 123: 329-334. https://doi.org/10.1007/s00418-005-0769-2 ‍ ‍ ‍ ‍ ‍ ‍ ‍ ‍ ‍ ‍ ZjQcmQRYFpfptBannerStart
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Espada J, Juarranz A, Galaz S, Canete M, Villanueva A, Pacheco M, Stockert JC (2005) Non-aqueous permanent mounting for immunofluorescence microscopy. Histochem. Cell Biol. 123: 329-334.  https://doi.org/10.1007/s00418-005-0769-2<https://urldefense.com/v3/__https:/doi.org/10.1007/s00418-005-0769-2__;!!LQC6Cpwp!_WRTrDLz9A3_IM5hW4x-FiEeMCT3HXZL4Eu9Uqk_fZDst6ecNYyWKwri4FYY0-V7kXxI9w$>
A PDF can be downloaded from the Google Scholar entry for this article.

John Kiernan
London, Canada
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________________________________
From: Morken, Timothy via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Sent: May 28, 2021 11:31 AM
To: Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Subject: [Histonet] IF with permanent mounting media?

Has anyone tried using xylene/permanent mounting media for immunofluorescence stains? I had a question from a pathologist who wondered if we could do this. I have never heard of anyone doing it.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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