[Histonet] Cryostat

[Morken, Timothy]

Guess I missed your other questions. 

I think the fog mist (Cold D) may be better - it reaches all area of the chamber. UV light is shaded from some areas, though probably hits most areas you might be touching. 

The misting requires a specific solution, not just anything. Hydrogen peroxide is the disinfecting agent. 

Other features:
We have the vacutome on the original units but we never use it. We do all kidney and muscle sectioning and these small samples do not produce much debris. On our new replacement unit (to replace a 7-year old NX70) we opted out of the vacuum.  If using in a gross room/Or setting the vacuum may be very useful. It only adds a hose and suction manifold in the unit and does not get in the way of anything. 




Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-----Original Message-----
From: Morken, Timothy via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Wednesday, May 19, 2021 8:26 AM
To: Brittany Hethcox <bhethcox at hcmhcares.org>
Cc: Histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Cryostat

Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're reliable and easy to use. We have the elevator on ours and it makes a difference for different size people in the lab. It also can have a vapor disinfectant system which we use and it works great. 

We use ours for kidney and muscle cryosectioning, not gross room/OR sections but these would work fine for that. They have a lot of room inside for specimens. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center


-----Original Message-----
From: Brittany Hethcox via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, May 19, 2021 7:21 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cryostat

Hello everyone!

I am new to the Histology world. You all may know my previous coworker, Rhonda Ford. She officially retired and I am so happy for her! With that being said, I am in a pickle because the cryostat we had completely broke down. We were having issues with it during my training period, so I never officially got to use it. We are now looking for a new cryostat for our department. My supervisor is asking me about different models and features, but I don't know much about any of this. So I was hoping you all could help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically the "Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our purchase (e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

--
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

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