[Histonet] Question - Bone marrow core biopy specimens

[Victoria Baker]

Hi Richard!

That is the duplicate of what we do in our lab.  We were putting both the
core and the clot on the same slide for in house testing, but den outs we
sometimes have to split the specimen and only on a slide.

I have a question for you about ISH Kappa/Lambda.  What decal protocol do
you use and do you see edge non specific detection/substrate on your
slides?  We use the Roche Iview blue.

I hope what I said helps.

Vikki

On Mon, May 3, 2021 at 4:12 PM Cartun, Richard via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hello everyone:
>
> I'm wonder what your protocol is for cutting formalin-fixed,
> paraffin-embedded bone marrow core biopsy specimens?  Do you cut a limited
> number of slides upfront and then go back to the block if the pathologist
> requests histochemical stains or immunohistochemical tests?  We are
> implementing a protocol today where we will cut "15" slides upfront; 3 H&Es
> at different levels and 12 unstains for potential histochemical stains
> and/or IHC tests.  Are we crazy?
>
> Thank you, and I hope everyone is doing well.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic
> Proteomics Laboratory
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 972-1596 (Office)
> (860) 545-2204 (Fax)
> Richard.cartun at hhchealth.org<mailto:Richard.cartun at hhchealth.org>
>
>
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