[Histonet] Prussian Blue Reaction

[Mac Donald, Jennifer]

Thank you.

From: Jay Lundgren <jaylundgren at gmail.com>
Sent: Monday, June 7, 2021 1:35 PM
To: Mac Donald, Jennifer <jmacdonald at mtsac.edu>
Cc: John Kiernan <jkiernan at uwo.ca>; Gudrun Lang <gu.lang at gmx.at>; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Prussian Blue Reaction

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Supposed to be insoluble.  Try potassium permanganate followed by oxalaic acid.  But book says insoluble.

On Monday, June 7, 2021, Mac Donald, Jennifer via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:
The instrument malfunction and it was overstained.

From: John Kiernan <jkiernan at uwo.ca<mailto:jkiernan at uwo.ca>>
Sent: Monday, June 7, 2021 9:50 AM
To: Gudrun Lang <gu.lang at gmx.at<mailto:gu.lang at gmx.at>>; Mac Donald, Jennifer <jmacdonald at mtsac.edu<mailto:jmacdonald at mtsac.edu>>
Cc: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Prussian Blue Reaction

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Overstained? Doesn't that mean the tissue contains a lot of iron and you are seeing where it is - which was the reason for doing Prussian blue histochemistry. Gudrun Lang correctly says that mineral acids won't remove it. Oxalic acid is said to dissolve Prussian blue (? by chelation); I've never tried this. If it works, you will no longer see where the iron is. To see features other than the distribution of iron, why not just stain another section from the block with a general-purpose stain like Giemsa or H&E?
John Kiernan
London, Canada
= = =
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From: Mac Donald, Jennifer via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu><mailto:histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>>
Sent: June 7, 2021 12:47 AM
To: Gudrun Lang <gu.lang at gmx.at<mailto:gu.lang at gmx.at><mailto:gu.lang at gmx.at<mailto:gu.lang at gmx.at>>>
Cc: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu><mailto:histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu><mailto:histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>>
Subject: Re: [Histonet] Prussian Blue Reaction

The tissue was overstained and the blue was interfering with interpretation

-----Original Message-----
From: Gudrun Lang <gu.lang at gmx.at<mailto:gu.lang at gmx.at><mailto:gu.lang at gmx.at<mailto:gu.lang at gmx.at>>>
Sent: Sunday, June 6, 2021 2:18 AM
To: Mac Donald, Jennifer <jmacdonald at mtsac.edu<mailto:jmacdonald at mtsac.edu><mailto:jmacdonald at mtsac.edu<mailto:jmacdonald at mtsac.edu>>>
Cc: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu><mailto:histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Subject: AW: [Histonet] Prussian Blue Reaction

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Hi Jennifer,
Why do you want to reduce the staining?

I ask, because the impact of hydrochloric acid on the tissue may influence the following results anyway.
I think, that the prussian blue pigment cannot be removed in an easy way. It is resistent to solvents and mineral acids.
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-for-Solvent-Based-Inks.html

On the other hand, if the blue colour doesn't interfere with your following staining, you can try to simple make a "double stain".

Regards
Gudrun

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Von: Mac Donald, Jennifer via Histonet
[mailto:histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>]
Gesendet: Sonntag, 6. Juni 2021 06:34
An: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu><mailto:histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Betreff: [Histonet] Prussian Blue Reaction


Does anyone know of a way to remove/reduce the Prussian blue reaction?
Thanks,
Jennifer



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