[Histonet] Prussian Blue Reaction

[Jay Lundgren]

Supposed to be insoluble.  Try potassium permanganate followed by oxalaic
acid.  But book says insoluble.

On Monday, June 7, 2021, Mac Donald, Jennifer via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> The instrument malfunction and it was overstained.
>
> From: John Kiernan <jkiernan at uwo.ca>
> Sent: Monday, June 7, 2021 9:50 AM
> To: Gudrun Lang <gu.lang at gmx.at>; Mac Donald, Jennifer <
> jmacdonald at mtsac.edu>
> Cc: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Prussian Blue Reaction
>
>   EXTERNAL SENDER- Exercise caution with requests, links, and attachments.
> Overstained? Doesn't that mean the tissue contains a lot of iron and you
> are seeing where it is - which was the reason for doing Prussian blue
> histochemistry. Gudrun Lang correctly says that mineral acids won't remove
> it. Oxalic acid is said to dissolve Prussian blue (? by chelation); I've
> never tried this. If it works, you will no longer see where the iron is. To
> see features other than the distribution of iron, why not just stain
> another section from the block with a general-purpose stain like Giemsa or
> H&E?
> John Kiernan
> London, Canada
> = = =
> ________________________________
> From: Mac Donald, Jennifer via Histonet <histonet at lists.utsouthwestern.edu
> <mailto:histonet at lists.utsouthwestern.edu>>
> Sent: June 7, 2021 12:47 AM
> To: Gudrun Lang <gu.lang at gmx.at<mailto:gu.lang at gmx.at>>
> Cc: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.
> utsouthwestern.edu> <histonet at lists.utsouthwestern.edu<mailto:hist
> onet at lists.utsouthwestern.edu>>
> Subject: Re: [Histonet] Prussian Blue Reaction
>
> The tissue was overstained and the blue was interfering with interpretation
>
> -----Original Message-----
> From: Gudrun Lang <gu.lang at gmx.at<mailto:gu.lang at gmx.at>>
> Sent: Sunday, June 6, 2021 2:18 AM
> To: Mac Donald, Jennifer <jmacdonald at mtsac.edu<mailto:jmacdonald at mtsac.edu
> >>
> Cc: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.
> utsouthwestern.edu>
> Subject: AW: [Histonet] Prussian Blue Reaction
>
>   EXTERNAL SENDER- Exercise caution with requests, links, and attachments.
>
> Hi Jennifer,
> Why do you want to reduce the staining?
>
> I ask, because the impact of hydrochloric acid on the tissue may influence
> the following results anyway.
> I think, that the prussian blue pigment cannot be removed in an easy way.
> It is resistent to solvents and mineral acids.
> https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.
> epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian-
> Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%
> 7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688
> f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAw
> MDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=
> KjvijcfrVPGZKGsAn6qX5rMKtulHpmsAzqHEkwz%2B96Y%3D&reserved=0<
> https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.
> epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian-
> Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C2eeb915058594b1d14b508d929d4
> 55c4%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637586814671423758%
> 7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik
> 1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=xKy3v4qvO4spk0zrRxXWZfcsHWxbQC
> %2FIQ1FbpMB390Y%3D&reserved=0>
> -for-Solvent-Based-Inks.html
>
> On the other hand, if the blue colour doesn't interfere with your
> following staining, you can try to simple make a "double stain".
>
> Regards
> Gudrun
>
> -----Ursprüngliche Nachricht-----
> Von: Mac Donald, Jennifer via Histonet
> [mailto:histonet at lists.utsouthwestern.edu]
> Gesendet: Sonntag, 6. Juni 2021 06:34
> An: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.
> utsouthwestern.edu>
> Betreff: [Histonet] Prussian Blue Reaction
>
>
> Does anyone know of a way to remove/reduce the Prussian blue reaction?
> Thanks,
> Jennifer
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu
> >
> https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.
> utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&
> data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%
> 7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%
> 7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik
> 1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=gRrUmRDEU3BfcA0rEgQOgBvPHIQ05I
> RM6WozVZiiR1g%3D&reserved=0<https://nam12.safelinks.
> protection.outlook.com/?url=http%3A%2F%2Flists.
> utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%
> 7C01%7Cjmacdonald%40mtsac.edu%7C2eeb915058594b1d14b508d929d455c4%
> 7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637586814671433754%7CUnknown%
> 7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik
> 1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=1p0zF%2BbxdarMOeh8R%
> 2B2HN3JrGroGYcbuplEXFxO6y1s%3D&reserved=0>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu
> >
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
> https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.
> utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%
> 7C01%7Cjmacdonald%40mtsac.edu%7C2eeb915058594b1d14b508d929d455c4%
> 7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637586814671443749%7CUnknown%
> 7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik
> 1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=%2FoXTqTKQY18uiQ%2BldVcTbiTv%
> 2FA9bJGdcM4BfODypIC4%3D&reserved=0>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>