[Histonet] Prussian Blue Reaction

[John Kiernan]

Overstained? Doesn't that mean the tissue contains a lot of iron and you are seeing where it is - which was the reason for doing Prussian blue histochemistry. Gudrun Lang correctly says that mineral acids won't remove it. Oxalic acid is said to dissolve Prussian blue (? by chelation); I've never tried this. If it works, you will no longer see where the iron is. To see features other than the distribution of iron, why not just stain another section from the block with a general-purpose stain like Giemsa or H&E?
John Kiernan
London, Canada
= = =
________________________________
From: Mac Donald, Jennifer via Histonet <histonet at lists.utsouthwestern.edu>
Sent: June 7, 2021 12:47 AM
To: Gudrun Lang <gu.lang at gmx.at>
Cc: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Prussian Blue Reaction

The tissue was overstained and the blue was interfering with interpretation

-----Original Message-----
From: Gudrun Lang <gu.lang at gmx.at>
Sent: Sunday, June 6, 2021 2:18 AM
To: Mac Donald, Jennifer <jmacdonald at mtsac.edu>
Cc: histonet at lists.utsouthwestern.edu
Subject: AW: [Histonet] Prussian Blue Reaction

  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.

Hi Jennifer,
Why do you want to reduce the staining?

I ask, because the impact of hydrochloric acid on the tissue may influence the following results anyway.
I think, that the prussian blue pigment cannot be removed in an easy way. It is resistent to solvents and mineral acids.
https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian-Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=KjvijcfrVPGZKGsAn6qX5rMKtulHpmsAzqHEkwz%2B96Y%3D&reserved=0
-for-Solvent-Based-Inks.html

On the other hand, if the blue colour doesn't interfere with your following staining, you can try to simple make a "double stain".

Regards
Gudrun

-----Ursprüngliche Nachricht-----
Von: Mac Donald, Jennifer via Histonet
[mailto:histonet at lists.utsouthwestern.edu]
Gesendet: Sonntag, 6. Juni 2021 06:34
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] Prussian Blue Reaction


Does anyone know of a way to remove/reduce the Prussian blue reaction?
Thanks,
Jennifer



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