[Histonet] Prussian Blue Reaction

[Mac Donald, Jennifer]

The tissue was overstained and the blue was interfering with interpretation

-----Original Message-----
From: Gudrun Lang <gu.lang at gmx.at> 
Sent: Sunday, June 6, 2021 2:18 AM
To: Mac Donald, Jennifer <jmacdonald at mtsac.edu>
Cc: histonet at lists.utsouthwestern.edu
Subject: AW: [Histonet] Prussian Blue Reaction

  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.

Hi Jennifer,
Why do you want to reduce the staining?

I ask, because the impact of hydrochloric acid on the tissue may influence the following results anyway.
I think, that the prussian blue pigment cannot be removed in an easy way. It is resistent to solvents and mineral acids.
https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian-Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=KjvijcfrVPGZKGsAn6qX5rMKtulHpmsAzqHEkwz%2B96Y%3D&reserved=0
-for-Solvent-Based-Inks.html

On the other hand, if the blue colour doesn't interfere with your following staining, you can try to simple make a "double stain".

Regards
Gudrun

-----Ursprüngliche Nachricht-----
Von: Mac Donald, Jennifer via Histonet
[mailto:histonet at lists.utsouthwestern.edu]
Gesendet: Sonntag, 6. Juni 2021 06:34
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] Prussian Blue Reaction


Does anyone know of a way to remove/reduce the Prussian blue reaction?
Thanks,
Jennifer



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